What Is Lamin A Protein

In this case lamin a may be dialyzed into tris hepes or napi buffers with ph 8 0 8 5 and containing 250 mm nacl and 1 mm dtt in which most of the lamin protein should stay soluble at concentrations below 50 μg ml for several hours.

What is lamin a protein.

It is an essential scaffolding supporting component of the nuclear envelope which is the membrane that surrounds the nucleus. Mutations in lamin genes can result in several genetic laminopathies which may be life threatening. After farnesylation at the caax box motif. Laminin interestingly is in the shape of a cross see here in fact a quick internet search turns up multiple websites selling t shirts mousepads stickers coffee mugs and a host of other items with a picture of the laminin protein.

Lamins line the inside of the nuclear membrane where they provide a platform for the binding of proteins and chromatin and confer mechanical stability. Mutations in the lmna gene cause hutchinson gilford progeria syndrome the lmna gene provides instructions for making a protein called lamin a. It stays associated with the membrane through protein protein interactions of itself and other membrane associated proteins such as lap1. At ph and salt concentrations below this or at higher protein concentrations lamin a will tend to oligomerize.

Its initial form called prelamin a undergoes a complex series of steps that are necessary for the protein to be inserted into the lamina. 90 of hgps cases carry the lmna g608g ggc ggt mutation within exon 11 of lmna activating a splice donor site that results in production of a dominant negative form of lamin a protein denoted progerin. Its initial form called prelamin a undergoes a complex series of steps that are necessary for the protein to be inserted into the lamina. Lamin a consists of a central rod domain flanked by hydrophobic n and c terminal domains.

Lamin c does not have to undergo this processing before becoming part of the lamina. They have been implicated in a wide range of nuclear functions including higher order genome organization chromatin regulation transcription dna replication and. Lamin proteins are involved in the disassembling and reforming of the nuclear envelope during mitosis the positioning of nuclear pores and programmed cell death. Lamin c does not have to undergo this processing before becoming part of the lamina.

Lamin a is targeted to the nuclear membrane by an isoprenyl group but it is cleaved shortly after arriving at the membrane. This protein plays an important role in determining the shape of the nucleus within cells. Over the past few months we have received numerous questions concerning a popular christian icon the protein laminin. Depolymerization of the nuclear lamins leads to disintegration of the nuclear envelope.

The lamin a protein must be processed within the cell before becoming part of the lamina. The lamins are the major architectural proteins of the animal cell nucleus.